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Journal: Stem Cells Translational Medicine
Article Title: Extracellular vesicles derived from differentiated granulosa-like cells restore the ovarian function of rats with premature ovarian insufficiency
doi: 10.1093/stcltm/szaf081
Figure Lengend Snippet: Granulosa cell marker proteins in rUCMSC-derived granulosa-like cells. (A) rUCMSC and GLC cell morphology. (B) Real-time PCR was used to analyze the gene expression levels of the stem cell pluripotency marker Oct4; the specific precursor granulosa cell marker Foxl2; and the mature granulosa cell markers Amhr2 , Cyp19a1 , and Fshr during the differentiation of rUCMSCs into granulosa cells. The values are the means ± standard errors of the means (SEMs) ( n = 5). The control (Crl) cells were undifferentiated rUCMSCs (Day 0). Gapdh was used as an internal control for quantification. * P < 0.05; ** P < 0.01. (C) Immunofluorescence images of granulosa cell marker proteins and stem cell pluripotency marker proteins. Bar: 100 µm. (D) AMH and E2 hormone secretion level of differentiated and undifferentiated rUCMSC in vitro ( n = 3). Control (Crl) cells are undifferentiated rUCMSCs. * P < 0.05.
Article Snippet: The primary antibodies included FOXL2 (Cat. No. A16244; Rabbit; ABclonal), OCT4 (Cat. No. SC-5279; Mouse; Santa Cruz), AMHR2 (Cat. No. SC‐377413; Mouse; Santa Cruz),
Techniques: Marker, Derivative Assay, Real-time Polymerase Chain Reaction, Gene Expression, Control, Immunofluorescence, In Vitro